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Paingankar, Mandar S.

Monitoring of Dengue and Chikungunya Viruses in Field-Caught Aedes aegypti (Diptera: Culicidae) in Surat City, India

Abstract Views :267 | PDF Views:81

Authors

Mandar S. Paingankar ¹, Mangesh D. Gokhale ¹, Keshav G. Vaishnav ², Paresh S. Shah ³

Affiliations
1 Medical Entomology and Zoology Department, Microbial Containment Complex, National Institute of Virology, 130/1, Sus Road, Pashan, Pune 411 021, IN
2 Vector Borne Diseases Control Department, Surat Municipal Corporation, Surat 396 003, IN
3 Dengue Group, National Institute of Virology, 20/A, Ambedkar Road, Pune 411 001, IN

Source

Current Science, Vol 106, No 11 (2014), Pagination: 1559-1567

Abstract

A virological surveillance was carried out in various places of Surat Metropolitan Region (SMR) to monitor the chikungunya and dengue activity in Aedes aegypti populations. A total of 556 pools constituting 4918 Ae. aegypti mosquitoes were collected and processed in the laboratory for the detection of chikungunya virus (CHIKV) and dengue virus (DENV) using Immuno Fluorescence Assay (IFA) and reverse transcription polymerase chain reaction (RT-PCR). Thirteen pools were found positive for DENV and one pool was found positive for CHIKV. Molecular phylogeny results revealed that DENV-3 GIII, DENV-4 GI and CHIKV ECSA are circulating in Surat. Based on GIS information and climate conditions, the risk map for Ae. aegypti and DENV infections was predicted. Risk maps suggest that the central and western parts of Surat are at high risk of Ae. aegypti spread and associated DENV infection.

Keywords

Arboviruses, GIS Mapping, Phylogenetic Analysis, Risk Map, Surveillance.

Full Text

Assessment of qPCR, Nested RT-PCR and Elisa Techniques in Diagnosis of Chikungunya

DNA Methylation Changes in a Gene-Specific Manner in Different Developmental Stages of Drosophila melanogaster

Abstract Views :223 | PDF Views:84

Authors

Chitra S. Panikar ¹, Mandar S. Paingankar ¹, Saniya Deshmukh ¹, Varada Abhyankar ¹, Deepti D. Deobagkar ¹

Affiliations
1 Molecular Biology Research Laboratory, Centre for Advanced Studies, Department of Zoology, Savitribai Phule Pune University, Pune 411 007, IN

Source

Current Science, Vol 112, No 06 (2017), Pagination: 1165-1175

Abstract

Although genomic DNA of Drosophila melanogaster has been shown to contain little cytosine methylation, the distribution of this genome-wide methylation patterns in different life stages remains to be elucidated. We have developed an immunochemical method using cDNA microarray to assess methylation. In the present work, this methylation microarray method was employed to identify DNA methylation in and around the genes in different life stages of D. melanogaster. This led to the identification of methylated genes in three stages of D. melanogaster, viz. embryo, pupa and adult. It is noteworthy that there was differential methylation in genes in different life cycle stages. Remarkably, a few functional annotation clusters showed negative correlation between transcription of a particular gene and its methylation status. In this analysis, some of the genes attributed to characteristic biological processes of particular life stage in D. melanogaster were found to be methylated in other life stages. Our analysis while providing a methylation map also suggests that gene-specific DNA methylation is altered during the life cycle stages of D. melanogaster.

Keywords

Developmental Regulation, DNA Methylation, Drosophila Development, Epigenetics, Gene-Specific Methylation, 5 Methyl Cytosine.

Full Text

References

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Pollution and Environmental Stressors Modulate the Microbiome in Estuarine Mangroves:A Metagenome Analysis

Abstract Views :217 | PDF Views:71

Authors

Mandar S. Paingankar ¹, Deepti D. Deobagkar ¹

Affiliations
1 Molecular Biology Research Laboratory, Centre for Advanced Studies, Department of Zoology, Savitribai Phule Pune University, Pune 411 007, IN

Source

Current Science, Vol 115, No 8 (2018), Pagination: 1525-1535

Abstract

The microbial communities of mangroves which form important links in elemental cycling and bioremediation have not been elucidated in most parts of the world. Due to urbanization and deforestation mangroves are also under threat. In the present study, high throughput next generation sequencing technology, based on 16S rRNA amplicon analysis using Illumina platform, was employed to unravel the microbial diversity present in different mangrove areas in the west coast of India. It could be seen that in mangroves, Proteobacteria and Bacteroidetes were most common, followed by taxon such as Firmicutes, Spirochaetes, Chloroflexi and Verrucomicrobia. In proteobacteria group, Gammaproteobacteria, Alphaproteobacteria and Deltaproteobacteria were most abundant. Interestingly, bacteria having the capacity to utilize sulphate were present along with methanogens in all samples, suggesting that anaerobic and sulphur- based metabolic pathways play an important role in these mangrove ecosystems. The differences in bacterial diversity can be partly attributed to biotic and abiotic factors such as physico-chemical characteristics of the samples, geographical location and natural and human-induced changes in the locality. The metagenomics analysis of mangrove sediment samples has helped in elucidating the baseline data on bacterial diversity along mangroves in Maharashtra along the west coast of India and can provide pointers for effective measures of conservation.

Keywords

Anthropogenic Stressors, Bacterial Communities, Metagenomics, Microbial Ecology, Pollution.

Full Text

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Microbial Diversity Analysis in the Oxygen Minimum Zones of the Arabian Sea using Metagenomics Approach

Abstract Views :276 | PDF Views:91

Authors

Mandar S. Paingankar ¹, Kedar Ahire ¹, Pawan Mishra ¹, Shriram Rajpathak ¹, Deepti D. Deobagkar ²

Affiliations
1 Molecular Biology Research Laboratory, Centre for Advanced Studies, Department of Zoology, Savitribai Phule Pune University, Pune 411 007, IN
2 ISRO Chair Professor, ISRO Space and Techn ology Cell, Savitribai Phule Pune University, Pune 411 007, IN

Source

Current Science, Vol 118, No 7 (2020), Pagination: 1042-1051

Abstract

Large oxygen-depleted areas known as oxygen minimum zones (OMZs) have been reported from the Arabian Sea, and recent reports indicate that these areas are expanding at an alarming rate. In marine waters, oxygen depletion may also be related to global warming and temperature rise. The acidification and deoxygenation due to OMZs can lead to major consequences wherein the plants, fish and other biota will struggle to survive in the ecosystem. The present study has identified the microbial community structure using next generation sequencing-based metagenomics analysis in water sa mples collected at different depths from the OMZs and non-OMZs of the Arabian Sea. Environmental variables such as depth, site of collection and oxygen concentration might influence species richness and evenness among microbial communities in these locations. Our observations suggest that population dynamics of microbes consisting of nitrate reducers accompanied by sulphate reducers and sulphur oxidizers influences the interconnected geoche mical cycles of OMZs. In addition to providing baseline data related to the diversity and microbial community dynamics in waters in the OMZs; such analysis can provide insight into processes regulating productivity and ecological co mmunity structure of the ocean.

Keywords

Bacterial Diversity, Metagenomics, Microbial Communities, Oxygen Minimum Zones.

Full Text

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